Fatty tissue – MS Imaging

This topic has 1 reply, 2 voices, and was last updated 16 years, 4 months ago by Simona Francese.

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July 12, 2007 at 1:40 am #1142

Richard Goodwin
Participant

Just wondering if anyone has any advice on imaging fatty tissue.

I’m washing sections mounted in ice to hold structure (-35oC chamber) and to avoid OCT – I am able to get some poor quality sections but with some structure…however when I wash (to remove ice crystals off plate and near tissue) I use 70% ethanol then 100% ethanol.

This destroys the sections and coagulates the fatty tissue. I’m interested in biomarkers in the 1-30kDa range.

anyone using fatty tissues have any advice?

Cheers

R.

July 10, 2008 at 3:17 pm #1232

Simona Francese
Participant

Hi,
I did work with pretty fatty tissue (skin cancer biopsy and pig ears). I did not mount them in ice so my situation would be different from yours.
The only thing I can say is that in my case, I set the cryostat at -30 and I cut the section on the opposite side of where most of the fat was.
Once the slice was mounted either on a tlc foil or on the target plate, I used gentle washing inclining the plate and with the aid of a syringe. Using chloroform is very effective for lipid removal.
I hope this helps a bit
Simona
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