anomaly between raw and processed data – MS Imaging

This topic has 1 reply, 1 voice, and was last updated 16 years, 7 months ago by Markus Stoeckli.

Viewing 2 posts - 1 through 2 (of 2 total)

Author

Posts
February 25, 2008 at 5:57 pm #1209

Anonymous
Guest

It seems I have encountered an aberration between the raw data (spectra at XY-coordinates) and the data from the .img file, generated from the raw data with AnalyzeThis!.

When I compare an individual spectrum at a certain set of coordinates (raw) with the spectrum shown by the plot function in BioMap, there seems to be a difference of mass between the peak signals. At 3 kDa it’s about 7-9 Da, but at 5,5 kDa the difference builds up untill 20 Da.

Has anyone here experienced this before? Or any idea what I could do to solve this problem?

March 7, 2008 at 1:32 pm #1215

Markus Stoeckli
Moderator

Based on your observation, I guess that the routine for writing the t2m-file has some flaws. The BioMap import routine would not generate the observed mass shift. This issue is best paced with the team who built the acquisition software.

Markus
Author

Posts

Viewing 2 posts - 1 through 2 (of 2 total)

You must be logged in to reply to this topic.