Yes, it works. We use it in our lab and it’s relatively robust and reproducible. In a typical coating we spray 8 ml of saturated CHCA solution in 50:50 ACN/0.1%TFA using a TLC sprayer. The authors state:[quote] The sample is allowed to dry for 1-2 min before the next coating cycle[/quote]
IMHO it is a requirement to never let the sample completely dry in between spray cycles ❗ .
Markus, could you elaborate on the flow rate during spraying, and what kind of surface is covered by the 8 mL of matrix solution?
Currently we use a home-built nebulizer, but only spray at 40?L/min because at higher flow rates the sections get too wet and the liquid is blown laterally over the sections due to the gas flow.
I can only estimate the flow rate of of our spray since we use a TLC sprayer operated by Bernoulli’s principle. We ajdust the spray to form a fine mist setting the pressure to approx 0.8 bar. This results in an estimated flow of 1 ml/min. We spray 8 ml in 30 minutes, with cycles of 10 s spray and 30 s pause.
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