Normalizing baselines

[Markus Stoeckli]

Yes, BioMap allows to apply a baseline correction to a full data set, but no, I personally don’t think that it works well on MALDI TOF data. Here are the starting points if you want to explore it:

Open a data set, open a Plot Tool window, select Config – Baseline Correction. In there play with the parameter till you have a found optimal conditions. Note the parameters, close the Plot Tool window and select Tools – Calculation – Baseline Correction in the BioMap main app. Enter the parameters and apply.

Good luck! Please let us know in this forum if you are successful with your data.

Markus

[Alia Schneider]

I’m still practicing with the demo rat in BioMap.
When I try to apply baseline correction
(scan from datamanager: Plot Tool > Config > Options > Filter Tab > Baseline correction > yes / or /
Plot Tool > Config > Baseline correction / or /
Tools > Calculation > Baseline Correction)
I always get the same error message window.
I add a screenshot.
Is it about the scan or do I miss sth.?

Thank you very much in advance.

Attachments:

• Error_BC.JPG
• Error_BC1.JPG

[Alia Schneider]

here the error window [img size=346]http://www.maldi-msi.org/images/fbfiles/images/Error_BC1.JPG[/img]

Attachments:

• Error_BC1.JPG

[Markus Stoeckli]

[b]schneial wrote:[/b]
[quote]When I try to apply baseline correction (scan from datamanager: Plot Tool > Config > Options > Filter Tab > Baseline correction > yes / or / Plot Tool > Config > Baseline correction / or / Tools > Calculation > Baseline Correction) I always get the same error message window. Is it about the scan? [/quote]

Uh, the demo data set does not have enough points… this is why it fails… I just tested it with 100 m/z points and it worked.

[Alia Schneider]

Ok thank you Markus. =)

[Author]

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