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Diesel
ModeratorNo, I’m sorry.
Maybe in the near future.Dieter
ModeratorHi Jon!
The [b]original file size should be [/b]1680 x 193 x 2551 x 2 = [b]1’654’272’482 bytes [/b]or 1’615’500 kB or 1577.6 MB !!!
And [b]not 217’799’278 bytes[/b]….
Maybe MMSIT stopped because the harddisk was full or something similar? This is also a possible answer for Error 2.This is in my opinion the main cause.
As another information: Error 1 ("Unable to allocate memory: to make array.") means you have not enough RAM in your PC. As a guiding principle: minimum two times of .img size
Hope you’ll find out, what the problem is/was.
Greets,
DieterModeratorHi Jon!
Which Biomap version do you use?
Dieter
ModeratorHi!
For BioMap you’ll need to transform your data to the Analyze Image 7.5 data format (Mayo Foundation, Rochester, MN).
You can check some publications (e.g. Stoeckli et al. 2002) and/or this [url=http://www.maldi-msi.org/index.php?option=com_joomlaboard&Itemid=29&func=view&id=16&catid=2]thread[/url].Dieter
ModeratorHi Matthias!
Sounds familar. :laugh: It’s the well known problem of more than 32768 data points of .img files in older versions of BioMap which work internally with 16-bit signed integers.
The newest version 3.7.5.2 solves this problem (see in NEWS, 08.01.2007).Regards,
DieterModeratorOpen your MSI and extract one image with your reference mass (e.g. matrix peak or a compound added to the matrix). Use ‘Tools-Calculation-Divide’ for normalizing the whole data set with your reference image.
ModeratorHi Bill!
v4.1/4.3 you should get from your local ABI dealer. Maybe only as an upgrade?
Data Explorer doesn’t care on which MALDI it is running. You can use v4.5 or v4.6 also for the DE-STR. But I can’t say if it’s running on NT 4.0 or not. Try it!
We run v4.5 on XP (DE-STR) and v4.6 on Win2000 (4700 TOF-TOF) and XP.Diesel
ModeratorI tried to retrace the error you decribed. I could [b]not[/b] get the error message with…
[ul]· a wrong (for BioMap unreadable) filename (e.g. space or dash) of the TIF
· different image compression the TIF with LZW, JPG or ZIP compressor (btw in BioMap is a misinterpretation of JPG compressed TIFs
· different byte order (IBM PC/Macintosh)
[/ul]
Maybe this helps you something
DieterModeratorLike already mentioned: I think the mass spectra contains more than 32768 data points. Check your acquisition method or .dat-file if this is true.
Dieter
ModeratorI’m wondering me too. But I don’t care: the signal intensity is many-fold higher than without sputtered gold. 😀
If anybody has a good theory … let us know.Dieter
ModeratorAdditionally I want to remark that we are using 8ml for coating 4 MALDI plates (40x40mm) at the same time. So for one of these plates approx. 2ml.
Dieter
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