Thanks for the reply. It does clarify some confusions. But it still doesn’t solve the problem. Since the intersted peak is stronger than the reference peak (such as matrix peak) in some location of image, the quotient will then be larger than 1. However the output window only display from 0 to 1. And those area (relative intensity higher than 1) will be display as dark area (low density area). How can I adjuste it?
I tried to slected one image window (m/z 190 for CHCA) as reference to divide other windows(other m/z) in a scan. But I found the resulted window didn’t change much compare with original one. But the resulted CHCA window (m/z 190) is more confusing. After normalized by itself, a uniform image is expected. But the resulted image is still similar with the original window (before normalization). Need help. Thanks