[sclerens]

Hi there
did you activate the programs on this website?
From memory the code is basically generated from a unique computer identifier, the website then performs a certain transformation on the code and this you have to enter in the software.

cheers
stefan

[sclerens]

Hi

You may want to check the regional settings on the PC, the decimal separator should be a point, not a comma.
Apart from that, the values are of course on the large side, I wouldn’t want to spot an attomole of that on my Maldi plate ;). Very likely something else is wrong – I’ll contact you on your gmail address so you can send me a few pixels of your experiment and I will have a look.

stefan

[sclerens]

Hi
terribly sorry, I am aware of this bug and it has been fixed – the new version will be uploaded shortly!
Once again my apologies.

stefan

[sclerens]

Thank you very much Eric, that looks easy enough to implement. I’ll contact you offline so you can test the new version before we publish it.

stefan

[sclerens]

Yep that sounds familiar :), it is a bug in the peak intensity conversion in Analyze This – apparently it only shows up when working with smoothed or background subtracted spectra.
I’m currently finalising an updated version of Analyze This in which the bug is fixed and hope to post this to this website in the following week; in the meantime you might try converting non-manipulated spectra and see if that helps.

cheers,
stefan

[sclerens]

I agree, it might be either that, or the VideoOCX control that is included (although that one [i]should[/i] be NT compatible).

[sclerens]

Good idea! Count me in!

Stefan

[sclerens]

Markus, could you elaborate on the flow rate during spraying, and what kind of surface is covered by the 8 mL of matrix solution?
Currently we use a home-built nebulizer, but only spray at 40?L/min because at higher flow rates the sections get too wet and the liquid is blown laterally over the sections due to the gas flow.

Stefan

[sclerens]

I did some experimentation with a 3×3 pixel .img file, with 4 intensity data points for each pixel. I used your [url=http://msi.stoeckli.net/forum/viewtopic.php?t=9]VB code[/url] to create the header.
You advised to put the intensity data in the first dimension, and this is also in the code for the header:
[code:1]ah.dime.dims(0) = 4
ah.dime.dims(1) = DPoints
ah.dime.dims(2) = XPoints
ah.dime.dims(3) = YPoints [/code:1]
However, when I do this, BioMap reads the 1st dim as X-data, the 2nd dim as Y-data and the 3rd dim as datapoints (Z). Hence I get a rectangular image on screen (4×3 pixels, with 3 slices) instead of a square (3×3 with 4 slices). Only when I put the datapoints in the 3rd dim, it looks OK. When dealing with real spectra, of course, it would be much nicer to keep the intensity data together as you suggest. What am I doing wrong?

[sclerens]

OK thanks! At first I was confused, but things are slowly starting to make sense now…

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