Walid M. Abdelmoula, Karolina Škrášková, Benjamin Balluff, Ricardo J. Carreira, Else A. Tolner, Boudewijn P. F. Lelieveldt, Laurens van der Maaten, Hans Morreau, Arn M. J. M. van den Maagdenberg, Ron M. A. Heeren, Liam A. McDonnell, and Jouke Dijkstra
Anal Chem. 2014 Sep 16;86(18):9204-11. Epub 2014 Aug 26.
The combination of mass spectrometry imaging and histology has proven a powerful approach for obtaining molecular signatures from specific cells/tissues of interest, whether to identify biomolecular changes associated with specific histopathological entities or to determine the amount of a drug in specific organs/compartments. Currently there is no software that is able to explicitly register mass spectrometry imaging data spanning different ionization techniques or mass analyzers. Accordingly, the full capabilities of mass spectrometry imaging are at present underexploited. Here we present a fully automated generic approach for registering mass spectrometry imaging data to histology and demonstrate its capabilities for multiple mass analyzers, multiple ionization sources, and multiple tissue types.