Automated, parallel mass spectrometry imaging and structural identification of lipids

Ellis SR, Paine MRL, Eijkel GB, Pauling JK, Husen P, Jervelund MW, Hermansson M, Ejsing CS, Heeren RMA

Nat Methods. 2018 May 21. doi: 10.1038/s41592-018-0010-6

We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.

https://www.ncbi.nlm.nih.gov/pubmed/29786091

 

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